2023 Fiscal Year Final Research Report
Construction of bionanomachines in which protein movements cooperate to express functions
Project/Area Number |
21K04850
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 28040:Nanobioscience-related
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Research Institution | Nara Institute of Science and Technology |
Principal Investigator |
Yamanaka Masaru 奈良先端科学技術大学院大学, 研究推進機構, URA (60632825)
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Co-Investigator(Kenkyū-buntansha) |
廣田 俊 奈良先端科学技術大学院大学, 先端科学技術研究科, 教授 (90283457)
松尾 貴史 奈良先端科学技術大学院大学, 先端科学技術研究科, 准教授 (50432521)
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Project Period (FY) |
2021-04-01 – 2024-03-31
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Keywords | ビルディングブロック / タンパク質デザイン / 構造領域交換 / タンパク質超分子 |
Outline of Final Research Achievements |
We conducted a detailed analysis of the behavior of protein supramolecules that had already been constructed at the start of the research, which incorporated movements (building block proteins with sensor proteins that dissociate from dimers to monomers by target molecules). We obtained insights into how the movements of the incorporated proteins are expressed in the overall movement of the supramolecule. Furthermore, we advanced the construction of a protein supramolecule structure design system that incorporates the structural prediction tool AlphaFold2, and we constructed protein supramolecules which have the three-dimensional structures with domain shuffling as designed. Throughout the research period, the accessibility and flexibility of designing protein supramolecules through domain shuffling improved dramatically.
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Free Research Field |
タンパク質工学
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Academic Significance and Societal Importance of the Research Achievements |
天然タンパク質超分子はナノスケールで駆動する分子機械=ナノマシンとして機能する。最近の深層学習技術により、誰でも自在にタンパク質超分子を設計できるような状況となったが、天然のタンパク質のように複雑に駆動するタンパク質超分子の自在設計には至っていない。本研究の成果は、人工タンパク質超分子に天然タンパク質のようなナノマシンとして複雑に駆動する性質を付与する分子設計の一助となる。
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