Development of active perfusion system where minute magnetic-drive parts are functionally distributed inside regenerated tissues

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K04853
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 28050:Nano/micro-systems-related
• Research Institution: Yamagata University
• Principal Investigator: INOUE Kenji 山形大学, 大学院理工学研究科, 教授 (40203228)
• Co-Investigator(Kenkyū-buntansha): 馮 忠剛 山形大学, 大学院理工学研究科, 教授 (10332545)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 再生組織 / 灌流 / 磁気駆動 / 細胞培養 / 精密ステージ

Outline of Final Research Achievements

An active perfusion system for regenerated tissues is proposed. Minute magnetic-drive parts which repeat expansion and contraction and serve as pumps are developed. The parts are distributed inside the tissues and driven by external magnetic field. The system actively generates and controls perfusion of culture solution inside the tissues.
Cell culture experiments are conducted. Collagen gel is populated with fibroblasts as tissue model, and some minute magnetic-drive parts are embedded in the gel. These are dipped into culture solution in a culture dish. By applying external magnetic field periodically, perfusion of culture solution is generated, and cell proliferation and activation are promoted. The results show that the proposed method is effective for cell culture.

Free Research Field

ロボット工学

Academic Significance and Societal Importance of the Research Achievements

iPS細胞を用いた疾病のモデリングと創薬スクリーニングには、再生医療に匹敵する重要性と市場価値があると言われている。しかし、３次元再生組織を構築する際に、組織深部にある細胞に栄養を供給できなければ、生体に近い組織の状態を再現できず、疾病モデリングや創薬スクリーニングの有効性と特異性が損なわれてしまう。
本研究の成果により、組織外部からポンプなどで内部に培養液を流し込む従来法では困難だった、組織全体にわたる栄養供給が可能となり、疾病モデリングと創薬スクリーニングの分野に大きく貢献できる。