2023 Fiscal Year Final Research Report
Probing conformational changes in protein reaction sites via Raman optical activity
| Project/Area Number |
21K04997
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 32010:Fundamental physical chemistry-related
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| Research Institution | Saga University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | ラマン光学活性 / 光受容タンパク質 / 低温分光 |
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| Outline of Final Research Achievements |
It is proteins that perform diverse chemical reactions in living organisms. In proteins, the visualization of three-dimensional structures of reacting molecules is one of the most important challenge. This research aimed to build up a new method using Raman optical activity (ROA) spectroscopy, which is very sensitive to three-dimensional (3D) structure of molecules, to track the biochemical reactions.
We chose the photoreceptors of micro-organisms, such as microbial rhodopsins and photoactive yellow proteins, for the target of our study and planned to measure ROA of their photointermediates trapped at low temperature. As the first step for this ROA measurement, we measured the low-temperature Raman spectra of the photointermediates of various photoreceptors and analyzed their structural changes in the photoreactions. Then, we extended our this Raman spectrometer to the low-temperature ROA spectrometer to obtain the three-dimensional structure information of photointermediates.
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| Free Research Field |
物理化学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で実施した光受容タンパク質の低温ラマン測定は良質な分子のスペクトルを得られる方法でありながら、世界的にみても同様の手法を用いる研究グループがほとんど無い。そのため、独自性ある構造情報に基づいた研究成果を発信することができる。さらに、これを発展させた低温ラマン光学活性(ROA)分光法は、今日広く利用されるどの分光手法でも測定ができない、溶液中のタンパク質の反応サイトの立体構造変化を捉えられる方法となる。本研究はタンパク質研究において"今まで見えなかった構造を観測する"展開を開拓する取り組みである
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