Analysis of mass transfer in pores related to chromatographic separation processes by single microparticle measurement method

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K05107
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 34020:Analytical chemistry-related
• Research Institution: University of Tsukuba
• Principal Investigator: NAKATANI Kiyoharu 筑波大学, 数理物質系, 教授 (00250415)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 細孔内拡散 / 吸脱着 / シリカゲル / ODSシリカゲル / 顕微分光法 / クロマトグラフィー

Outline of Final Research Achievements

Diffusion in pores of a small solute in an ODS silica gel/organic solvent-water system and a polymer in a silica gel/water system, related to chromatographic separation processes, has been analyzed by single microparticle manipulation and microspectroscopy techniques. Intraparticle diffusion of coumarin 102 in acetonitrile-water was limited by surface diffusion while that in 1-butanol-water was governed by both pore and surface diffusions. Adsorption/desorption rates of myoglobin on pore walls in silica gel were extremely slow. Therefore, intraparticle mass transfer of the biopolymer was simulated on the basis of the pore and surface diffusion model as the slow adsorption/desorption rates.

Free Research Field

分析化学

Academic Significance and Societal Importance of the Research Achievements

微粒子細孔内物質移動は，クロマトグラフィーやドラッグデリバリーシステム，触媒などに関係し，その速度論的機構解明はこれらシステム・材料開発に不可欠である。本研究では，特にクロマトグラフィーで用いられる粒子，測定条件での細孔内物質移動を直接測定できるようになり，低分子と高分子の物質移動の違いについて明らかにした。本結果と研究手法は，分析化学，界面化学，材料化学の分野の研究に有用な知見を与えたものと考えられる。