Development of light-dependent apoptosis induction by lactosome-loaded pre-miR-664a

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K05277
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 37010:Bio-related chemistry
• Research Institution: Okayama University
• Principal Investigator: Watanabe Kazunori 岡山大学, ヘルスシステム統合科学学域, 准教授 (70602027)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: pre-miR-664a / 光制御 / アポトーシス誘導

Outline of Final Research Achievements

Apoptosis induction methods are important in cancer therapy. We have reported that the combination of light-induced RNA delivery and pre-miR-664a, which induces apoptosis, can induce apoptosis in a light-dependent manner. However, the light-induced RNA delivery is difficult to use in vivo. Therefore, we attempted to develop a light-dependent apoptosis induction by loading pre-miR-664a and a photosensitizer onto lactosome that maintain high stability in vivo.
As a result, apoptosis was induced in a light-dependent manner by lactosome loaded with pre-miR-664a and a photosensitizer, and lactosome loaded with pre-miR-664a and photosensitizer showed higher apoptosis induction efficiency than those loaded with a photosensitizer alone.

Free Research Field

生体分子工学

Academic Significance and Societal Importance of the Research Achievements

高効率なアポトーシス誘導が可能なpre-miR-664aと光増感剤を担持したラクトソームの開発に成功した。今後、in vivo研究へと発展させていくことで、pre-miR-664aと光増感剤を担持したラクトソームを用いた抗がん剤へと開発していくと考えている。