Precise measurement of bioluminescence and chemiluminescence reaction quantum yield

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K05321
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 37030:Chemical biology-related
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Niwa Kazuki 国立研究開発法人産業技術総合研究所, 計量標準総合センター, 研究グループ長 (30443211)
• Co-Investigator(Kenkyū-buntansha): 山崎 太一 国立研究開発法人産業技術総合研究所, 計量標準総合センター, 主任研究員 (00462838)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 生物発光 / 量子収率 / 分光計測

Outline of Final Research Achievements

The quantum yield (Quantum Yield, QY) of a bioluminescent reaction, such as firefly, is an indicator of the photoproduction efficiency of the luminescent reaction. It is determined from the ratio of the number of reacted substrate molecules to the number of emitted photons. However, conventional measurement methods have had problems with low luminescence levels or dark reaction systems. In this study, the quantitative accuracy of both the number of substrate molecules and the number of photons was re-examined using the knowledge of the national measurement standards by the NMIJ/AIST. Furthermore, a system for quantifying the number of photons produced while monitoring the remaining amount of substrate by HPLC is under construction. In the future, we plan to measure and evaluate dark luminescence reaction systems, which have been difficult to evaluate QY.

Free Research Field

精密分光計測

Academic Significance and Societal Importance of the Research Achievements

発光反応は臨床検査や環境汚染物質分析などで広く応用されており、現在でも多様な発光反応物質の開発や、やそれらの応用方法の開発に向けた研究が展開されている。量子収率は反応の明るさを示す重要な指標であるが、発光量が少ない暗い反応系では測定できたいという問題があった。本研究によりこれまでQYの評価が困難であった暗い発光反応系についても量子収率が測定できるようになり、効率的な発光物質の開発に貢献できるものとなる。