Functional analysis of LjSYP132s, a regulator of vesicular transport, in nodule and seed formation.

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K05327
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38010:Plant nutrition and soil science-related
• Research Institution: Kagawa University
• Principal Investigator: Nomura Mika 香川大学, 農学部, 教授 (50294749)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: SNARE / Nodule / Legume / nitrogen fixation / Lotus japonicus

Outline of Final Research Achievements

In this project, we focused on the splice variants of LjSYP132s and investigated their subcellular localization and functional complementation, and determined the cis region important for selective splicing, aiming to elucidate the whole picture of selective splicing, whose regulatory mechanism is still unknown. The results of the analysis using the LjSYP132bLORE1 mutant showed that the expression of LjSYP132a increased when the expression of LjSYP132b decreased. The localization of LjSYP132a and LjSYP132b in FLAG-tagged cells was also confirmed, indicating that neither LjSYP132a nor LjSYP132b changes localization.

Free Research Field

植物栄養学

Academic Significance and Societal Importance of the Research Achievements

本申請課題では、LjSYP132sのスプライスバリアントに着目し、それぞれの細胞内局在性と機能相補性を調べ、選択的スプライシングに重要なシス領域を決定することで未だその制御機構が不明な選択的スプライシングの実体についてその全容を解明することを目標としている。根粒形成と花粉管伸長の共通性が明らかになれば、根粒形成メカニズムの進化のルーツを解き明かすことができると期待される。