2023 Fiscal Year Annual Research Report
Spatiotemporal mapping of gene expression to reveal mechanisms of cellular differentiation in E. coli biofilms
| Project/Area Number |
21K05341
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| Research Institution | Kyoto University |
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Principal Investigator |
Robert Martin 京都大学, 薬学研究科, 特定准教授 (90365487)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | biofilm / E. coli / imaging / gene expression / proteome analysis / cell motility / acid fermentation |
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| Outline of Annual Research Achievements |
1) We completed the spatial analysis of protein expression. The results revealed specific enrichment of biological functions into different parts of the biofilm and hints of functional differentiation
2) We designed, developed and benched-marked a new automated and multi-mode imaging system to monitor the growth of biofilms, their cell density, surface morphology, and fluorescence in two different colors
3) During growth of biofilms on LB containing glucose we revealed a spatio-temporal metabolic shift that influences growth rate and three-dimensional structure.
4) We found that an expression of an uncharacterized cell-death related gene can affect biofilm 3D structure. This phenomenon seems to occur in a calcium-dependent process and affects cell density and biofilm morphology.
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| Remarks |
This site summarizes the project, its aims, and its outcomes.
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