2023 Fiscal Year Final Research Report
Spatiotemporal mapping of gene expression to reveal mechanisms of cellular differentiation in E. coli biofilms
| Project/Area Number |
21K05341
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38020:Applied microbiology-related
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| Research Institution | Kyoto University |
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Principal Investigator |
ROBERT Martin 京都大学, 薬学研究科, 特定准教授 (90365487)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | biofilm / E. coli / gene expression / imaging / differentation / bacterial growth / proteomics / stress resistance |
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| Outline of Final Research Achievements |
This project was about examining functional differentiation in E. coli biofilms by monitoring growth, morphology and gene expression during development.
We found that in complex media glucose affects biofilm formation resulting in structural differences as well as functional differences linked to metabolism and response to acid stress during growth on glucose. Differential spatio-temporal expression was observed both with fluorescent reporters and proteome analysis. Morphological differences arise from changes in surface hardness as well as local nutritional conditions. We observed changes in gene expression activity and protein levels related to motility, acid resistance, metabolism, biofilm formation and protein synthesis. As planned, we provided novel evidence for spatiotemporal differentiation in activity in different parts of the biofilm (top-bottom and center-edge axis) and over time (biofilm growth and aging) that suggest co-existence and interactions between these populations.
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| Free Research Field |
microbiology 微生物学
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| Academic Significance and Societal Importance of the Research Achievements |
この結果はバイオフィルムの成長と発達に対するグルコースの重要な影響を浮き彫りにした。 また、環境条件や薬剤などに対する耐性を試験するために使用できる単純な生物学的モデルにおける、多細胞の増殖と分化に関する基本的な洞察を提供する。バイオフィルムの生存と形成に重要な耐酸性、マトリックス産生、運動性遺伝子に関連した明らかな変化は、バイオフィルムの形成を制御するための新たなターゲットとなる可能性を示唆している。加えて、我々は関連分野の他の研究者にも有用な、オープンソースタイプの汎用的で安価なマルチモードイメージングシステムを開発した。
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