Search and functional analysis of maturation genes for syntrophic propionate oxidation using genomic information

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K05343
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38020:Applied microbiology-related
• Research Institution: Yamaguchi University
• Principal Investigator: Kosaka Tomoyuki 山口大学, 大学研究推進機構, 准教授 (70500453)
• Co-Investigator(Kenkyū-buntansha): 松谷 峰之介 東京農業大学, その他部局等, 准教授 (70380558)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: ゲノム情報 / 成熟化因子 / 大腸菌 / 異種発現 / プロピオン酸酸化細菌 / コハク酸脱水素酵素 / ヒドロゲナーゼ / 膜タンパク質

Outline of Final Research Achievements

We have constructed the procedure for visualizing related genes for protein complex functions from genomic information, which provides the way to explore the required genes for function when the function of it is unknown. Although the functional analysis of candidate maturation genes was not so successful, the function of endpeptidase of propionate oxidizing bacteria for [NiFe]-hydrogenase maturation in Escherichia coli cell was revealed by our analysis. In addition, the improvement of the method of expression regulation of these maturation genes was shown to be the key to their analysis. Furthermore, the analysis of non-functional membrane-protein complexes of other microorganisms, such as succinate dehydrogenase and [NiFe]-hydrogenase, in E. coli should improve its expression before considering its activity, which was improved by genomic modification of expressing E. coli cells.

Free Research Field

微生物機能学

Academic Significance and Societal Importance of the Research Achievements

大腸菌での異種遺伝子発現は数多く試みられてきているが、機能がほとんど見られない場合は改善方法が明快でないため研究の道筋が立たない。今回ゲノムの情報から特定の遺伝子の機能化に必要な関連遺伝子を示唆することが可能であり、その可視化方法を構築したことから、ゲノム情報を基にすれば異種細胞での発現遺伝子の機能化の期待値を上げることが可能である。つまりは、活用されていない、そして機能が未知のままである遺伝子の活用の道筋を立てることを可能にする成果である。