2023 Fiscal Year Final Research Report
Structural and functional analysis of a peptide ABC transporter for paraben
| Project/Area Number |
21K05351
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38020:Applied microbiology-related
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| Research Institution | Tokyo University of Agriculture |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 基質認識 / Ｘ線結晶構造解析 |
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| Outline of Final Research Achievements |
It was revealed that parabens are natural substrates for hydrazidase, which is isolated from Microbacterium hydrocarbonoxydans grown with hydrazide compounds as sole carbon source. Growth experiments and gene expression analysis of the bacteria also indicated that parabens are catabolized by the bacteria. Furthermore, a homologue of the enzyme was isolated from another actinomycete, and its function and structure were analyzed. Although the homologue also catalyzed parabens as substrates, differences were observed in the activity of the two enzymes, which was attributed to structural differences in the entrance region of the active sites. On the other hand, the analysis of the transporter that takes up parabens requires further investigation to obtain the recombinant protein.
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| Free Research Field |
蛋白質科学
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| Academic Significance and Societal Importance of the Research Achievements |
化合物を資化する仕組みにおいてトランスポーターと分解酵素の役割は重要であるが、データベースの情報のみでは、本来の基質を正確に予測することは容易ではない。今回、立体構造解析等をもとにパラベンを代謝する最初の段階の仕組みを明らかにした。パラベンは化粧品や食品の保存料として使われている一方、内分泌系への懸念もいわれる。またパラベンが植物体の構成成分として存在するという報告もある。パラベンを触媒する蛋白質、また資化する菌は本研究が初めての報告であり、分子レベルでは蛋白質機能解析に新たな知見を加えた点、微生物としてはバイオレメディエーションへの応用などが考えられる研究成果である。
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