Research and development of a novel platform of cell-plastics directly to use its metabolites

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K05371
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38020:Applied microbiology-related
• Research Institution: Tokyo University of Technology
• Principal Investigator: NAKANISHI Akihito 東京工科大学, 応用生物学部, 助教 (60640977)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 細胞プラスチックス / 光合成微生物 / 細胞内容物

Outline of Final Research Achievements

Cell viability and leakage of the cell-contents such as lipids and proteins were analyzed to evaluate the effect of the cell death by not only over time during culture but also sterilization treatment with UV-C irradiation. As a result, the leakage of protein components was observed derived from the cell death. In cell plastic fabrication, the proteins with long chains of α-helices in the outer structure could play an important role in the cell-attachment, meaning that the cells in protein-leaking phase could easily contact each other and fabricated. As a result of GC/MS analysis, the aroma component from the cell plastics was identified as the antibacterial substance cis-2-penten-1-ol. The above research results displayed the possibility of using green algae cells directly as a raw material for cell plastics and directly utilizing the metabolites produced by the cells.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

細胞カプセル技術については、必要な時期に細胞内容物を漏出させることが重要である。細胞死に際し、タンパク質成分の漏出が認められ、外郭構造にαヘリックスの長鎖長を有するタンパク質が細胞間の接合に重要な内容物であると同定されている。以上から、細胞同士を接合させやすく、成型しやすくなる状態の細胞を示した。また、細胞プラスチックスの香気成分を抗菌性物質として同定した。抗菌成分はプラスチックス利用において重要な性能であり、細胞プラスチックス利用における有意な成果だと考えた。従って、緑藻細胞を直接的な原料とし細胞の代謝産物の直接的な利用の可能性を示したことは代謝物の使用選択幅を広げた点で学術的に意義を持つ。