2023 Fiscal Year Final Research Report
Identification of green pigments of green lacewings and their biosynthesis pathways
| Project/Area Number |
21K05402
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38040:Bioorganic chemistry-related
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| Research Institution | Ehime University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | green pigments |
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| Outline of Final Research Achievements |
The green pigments contained in the body color of green lacewings, Crysoperla nipponensis, were investigated using MS, and biliverdin was identified as one of the green pigments. The sequences of the genes encoding the enzymes related in the biosynthesis of biliverdin were revealed, and the recombinant enzymes were overexpressed in E. coli to clarify the features of these enzymes. In addition, the mRNA levels were quantified using realtime PCR, and the suppression of the gene expression by the injection of the double strand RNAs. These results demonstrate that the green pigments contain not only biliverdin but also the other green pigment(s).
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| Free Research Field |
生物有機化学
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| Academic Significance and Societal Importance of the Research Achievements |
世界中で緑色の体色を持つ昆虫は数多く認められるが、その緑色の生合成経路や役割は未解明の点も多い。本研究でクサカゲロウを用いて得られた成果は、他の緑色昆虫と比較することにより、緑色色素が昆虫の生理、生態にどのように影響をおよぼしているのかを解き明かすうえで役立つことから、学術的意義は高い。また、得られた色素は食品添加物、着色料や塗料などの応用が期待でき、社会的に研究成果を還元できる可能性がある。
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