Analysis of the regulation system of sulfur metabolism in high sulfur-containing Japanese heirloom vegetables of the Brassicaceae family

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K05461
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38050:Food sciences-related
• Research Institution: Nihon University
• Principal Investigator: YAMAYA-ITO Hiroko 日本大学, 生物資源科学部, 准教授 (80648684)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: イオウ代謝 / 伝統野菜 / アブラナ科植物 / 機能性成分 / 酵素

Outline of Final Research Achievements

In order to cultivate vegetables with a high content of functional sulfur(S)-containing compounds, it is necessary to elucidate the activation mechanism of the organic S compound synthesis pathway. In this study, we classified Brassicaceae heirloom vegetables based on their S-absorption capacity, and compared the activity of key enzymes controlling organic S metabolism under the condition of increasing the rhizosphere sulfate concentration. In the cultivars with low S absorption capacity, the content of both inorganic and organic S was increased, and a correlation was observed between the enzyme ATPS which leads to organic S metabolism, and the enzyme APK which leads to the metabolism of oxidized sulfur-containing compounds. These results suggest that the cultivars with low S absorption capacity may be stored the surplus S as inorganic S in vacuoles and also distributed the surplus S to organic S, especially for oxidized sulfur-containing compounds.

Free Research Field

植物栄養学

Academic Significance and Societal Importance of the Research Achievements

機能性を持つ有機態イオウ（S）化合物の含有量を高めることができれば、摂食目的に適う機能性成分高含有野菜を育成することが可能となる。本課題では、アブラナ科植物はS吸収能により余剰Sの分配傾向が異なり、品種レベルで独自のS吸収・S代謝制御機構を有していることを明らかにした。さらに根域S濃度上昇に応答して有機態S化合物合成経路の鍵酵素が活性化され、有機態Sの割合が増加する品種の選抜に成功した。今後、選抜した品種における、S含有量の増加またはS機能性成分生合成経路が促進される時に増加する物質あるいは現象を解析することで、機能性成分高含有野菜の選抜マーカーとしての利用に繋げることができると考えられる。