Development of novel breeding materials using cytosol-localized isoforms

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K05531
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39010:Science in plant genetics and breeding-related
• Research Institution: Setsunan University
• Principal Investigator: Ushijima Tomokazu 摂南大学, 農学部, 准教授 (50815058)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 環境ストレス応答 / 細胞質局在型アイソフォーム / 光応答

Outline of Final Research Achievements

The purpose of this study was to generate overexpressors and deficient mutants of cytoplasm-localized isoforms produced by light-dependent transcription start site regulation in rice, to clarify the function of the cytoplasm-localized isoforms, and to evaluate these genes as breeding materials. As a result, we confirmed again that light-dependent transcriptional start site changes occur in rice. Therefore, we constructed a vector to analyze the cytoplasm-localized isoforms generated by this regulation. We were able to clone the candidate genes into the vector.

Free Research Field

遺伝育種科学

Academic Significance and Societal Importance of the Research Achievements

本研究は赤色光依存的に転写開始点の選択が変化し、その結果生じるタンパク質の局在が変化するという新しい知見に基づいて行った。その結果、イネにおいても当該制御で新奇の細胞質局在型タンパク質が生じることが再確認された。これらの細胞質局在型タンパク質は環境ストレスの低減に働くと考えられることから、当該制御で生じる細胞質局在型タンパク質の機能を明らかにすることで、環境変動に対する育種に有効な遺伝子の発見につながることが期待される。