2023 Fiscal Year Final Research Report
Study on unstable anthocyanin pigmentation genes and epigenetic mechanisms
| Project/Area Number |
21K05574
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 39030:Horticultural science-related
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| Research Institution | Kyoto University |
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Principal Investigator |
OHNO Sho 京都大学, 農学研究科, 助教 (10722001)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | トウガラシ / アントシアニン / エピジェネティクス / MYB転写因子 / レトロトランスポゾン / DNAメチル化 |
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| Outline of Final Research Achievements |
In this study, the mechanism of unstable anthocyanin pigmentation was analyzed in the UP line, which exhibits color variation in flowers due to lateral mutant of anthocyanin-accumulating chili pepper 'Peruvian Purple'. It was suggested that the gene responsible for the unstable anthocyanin pigmentation was mapped on an approximately 1 Mb region containing the positive anthocyanin regulating transcription factor CaMYBA. Since there was a correlation between anthocyanin pigmentation and DNA methylation in the 5'-terminal region of the retrotransposon LINE-1 inserted into CaMYBA, it was suggested that variations in DNA methylation in this region are involved in the unstable anthocyanin pigmentation of the UP line.
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| Free Research Field |
園芸科学
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| Academic Significance and Societal Importance of the Research Achievements |
園芸植物において，同じ遺伝背景を有しながらも異なる表現型を示すことが多くあり時には問題となるが，その多くで詳細なメカニズムは明らかになっていない．本課題ではそのモデルとして，突然変異により着色変動性を示すようになったトウガラシのUP系統の着色変動メカニズムを解析している．本研究によって，着色変動に関わると考えられるDNAメチル化領域を特定することができ，さらにMcrBC-PCRによる判別マーカーも作出できたことから，今後さらに研究を進めることで環境要因が及ぼすエピジェネティックな影響を解析できるようになったと考えている．
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