Genetic servey of escaped farmed red sea breamin major aquaculture area

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K05730
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 40030:Aquatic bioproduction science-related
• Research Institution: Nihon University
• Principal Investigator: SAWAYAMA Eitaro 日本大学, 生物資源科学部, 准教授 (70846071)
• Co-Investigator(Kenkyū-buntansha): 高木 基裕 愛媛大学, 南予水産研究センター, 教授 (70335892)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 養殖魚 / 逸出 / 遺伝的撹乱

Outline of Final Research Achievements

Red sea bream is a species for which intensive aquaculture and breeding have been extensively developed. In this study, we used this fish species as a model to monitor escapee around major aquaculture areas. Initially, to differentiate between farmed and wild fish, a single nucleotide polymorphism (SNP) marker was developed, and a molecular genetic method was established using this to identify escapee. An SNP panel was created using 249 SNP markers evenly selected from across the genome. Upon evaluating the efficacy of this SNP panel, approximately 200 SNPs were amplified with sufficient accuracy for analysis, allowing for the genetic discrimination between farmed and wild fish. Investigations using this SNP panel on escaped fish showed that escapes were frequently observed in aquaculture areas located inner bay.

Free Research Field

水産増殖

Academic Significance and Societal Importance of the Research Achievements

一連の研究により、マダイの個体識別を可能にする高感度DNAマーカーの開発に成功し、日本沿岸域のマダイの遺伝的多様性の現状や、逸出による遺伝的撹乱を明らかにできた。このことは、水産重要魚種の遺伝資源を保全する上での重要な基礎情報となるとともに、他魚種においても同様の技術を転用することが可能となるため、学術的意義は大きいと言える。また、逸出の現状を明らかにしたことで、今後の養殖管理についてのあり方を検討する知見としての意義も大きい。