2023 Fiscal Year Final Research Report
Development of a simplified method for species identification of Acropora corals by genomic hybridization
| Project/Area Number |
21K05734
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 40030:Aquatic bioproduction science-related
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| Research Institution | Kochi Gakuen University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
久保田 賢 高知大学, 教育研究部総合科学系黒潮圏科学部門, 教授 (00314980)
目崎 拓真 公益財団法人黒潮生物研究所, 研究部局, 研究所長 (20840482)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | FISH |
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| Outline of Final Research Achievements |
We have been clarifying the possibility that chromosome analysis by molecular cytogenetics may be effective as a third means of discrimination when molecular classification by morphological observation and DNA analysis makes it difficult to discriminate species.
In this study, we confirmed the usefulness of long-term cryopreserved embryos and attempted to detect differences between Acropora hyacinthus and either A. muricata or A. sp. Entaku by genomic hybridization. The quality of the preserved embryos, such as the clarity and length of the chromosome images, was maintained after 3 to 4 years of preservation, indicating no problems in the analysis. In each of the two stained images compared, signals indicating interspecific differences, possibly due to repetitive sequences, were detected.
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| Free Research Field |
分子細胞遺伝学
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| Academic Significance and Societal Importance of the Research Achievements |
様々な生物の生息場所を提供するサンゴ礁を形成する造礁性サンゴは，世界中で約800種に上る多様性を示す。その分類には，形態分類やDNA配列分子が用いられているが，種数が多く，広く観察されるミドリイシ属サンゴについては，水流や日照の強さなどの環境により，著しく異なる形状を示し，種同定を困難にしている。この属のサンゴは，環境保全目的の移植に高頻度で用いられるなど注目度が高いが，無計画な移植活動が取り返しのつかない遺伝的かく乱を招くとの指摘も多い。
本研究でゲノムハイブリダイゼーション法の有効性が示されたことから，より効果的な保全手法への改善が期待される。
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