2023 Fiscal Year Final Research Report
Development of a vaccine against Edwardsiellosis using MHC polymorphism as an adjuvant
| Project/Area Number |
21K05769
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 40040:Aquatic life science-related
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| Research Institution | Tokyo University of Marine Science and Technology |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | MHCの多様性 / アジュバント / 魚類ワクチン |
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| Outline of Final Research Achievements |
Vaccines against fish Edwardsiellosis in aquaculture have not been sufficiently effective because the bacterium is an intracellular parasitic pathogen. On the other hand, in this study, we were able to obtain a certain vaccine effect in ginbuna crusian carp by administering formalin killed bacteria by activating cell-mediated immunity using allogenic fish blood injection. In addition, the vaccine effect of FKC was obtained even in administration of red blood cells, which are thought to express only MHC class I. The effect was also observed when concurrent administration of allogenic blood cells and FKC was observed, suggesting that the work is relatively easy when practical application is considered.
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| Free Research Field |
魚病学
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| Academic Significance and Societal Importance of the Research Achievements |
特別な性質を持つ細胞内寄生菌によるエドワジェラ感染症は未だに魚類養殖業において猛威をふるっており、十分な予防効果が得られているワクチンの開発には成功していない。本研究では、魚個体間の主要組織適合抗原（MHC）の多型性に視点をおき、別個体に接種した血液を細胞性免疫活性化のアジュバントとして利用しようとする新しい試みである。
研究の結果、MHCの異なるギンブナの血液注射は、レシピエントにE. tardaホルマリン死菌体投与にするとエドワジエラ症に対して強い免疫を獲得し、攻撃試験において有意な死亡率の低下さた。また両注射を同時に行うことも可能で、作業が軽減できることが示唆された。
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