2023 Fiscal Year Final Research Report
Biosynthetic pathway of fucoxanthin in brown algae
Project/Area Number |
21K05773
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 40040:Aquatic life science-related
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Research Institution | Ishikawa Prefectural University |
Principal Investigator |
Takemura Miho 石川県立大学, 生物資源環境学部, 准教授 (20273857)
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Project Period (FY) |
2021-04-01 – 2024-03-31
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Keywords | 褐藻 / フコキサンチン / 大腸菌 |
Outline of Final Research Achievements |
In this study, upstream and downstream studies were conducted to elucidate the fucoxanthin biosynthesis pathway in brown algae. In the analysis of the upstream pathway, we found genes homologous to known plant carotenoid biosynthesis genes, PSY1,2, PDS1, ZISO1, ZDS1, CRTISO1,2, LCYb1, CYP97B1,2, ZEP1,2. For the downstream pathway, we searched for genes that might be involved in the biosynthetic reaction, but could not find any candidate genes. Functional analysis of a gene homologous to VDL1 in diatoms was also performed, but activity could not be detected. However, in the course of this analysis, it became possible for the first time to produce neoxanthin in E. coli.
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Free Research Field |
代謝工学
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Academic Significance and Societal Importance of the Research Achievements |
本研究により、褐藻(オキナワモズク)も、フコキサンチン生合成経路の上流では植物と同様の遺伝子の活性により同様のカロテノイド生合成経路を有していることが明らかとなった。また、下流では、珪藻と同様の遺伝子を有することが明らかとなったが、大腸菌での活性が非常に低いことが考えられた。しかしながら、珪藻の遺伝子を用いることによって、ネオキサンチンを大腸菌で生産することが初めて可能となり、今後のフコキサンチン生産研究にとって必要不可欠な成果を得ることができた。
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