Metabolomics-based production of high value-added sturgeon meats and eggs

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K05782
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 40040:Aquatic life science-related
• Research Institution: Hiroshima University
• Principal Investigator: Naganuma Takeshi 広島大学, 統合生命科学研究科(生), 教授 (70263738)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: チョウザメ / メタボライト / メタボローム / メタボロミクス

Outline of Final Research Achievements

In the first year, 10 fish in experimental and control areas were fed either a commercial rainbow trout diet or the same diet enriched with 5% lactic acid or 5% betaine. Blood samples underwent metabolomic analysis.
In the second year, lactic acid and betaine were administered, with blood collected twice and metabolomic analysis conducted. After the second blood collection, meat and fat tissues were gathered from both experimental and control areas for further metabolomic analysis.
Third-year food was supplemented with food industry by-products, such as "culture supernatant" from commercial lactic acid bacteria culture and "waste molasses" from sugar beet processing, in place of lactic acid and betaine. Samples of blood, meat, and fat were taken from experimental areas, and samples of waste molasses or lactobacillus culture supernatant were added before being subjected to metabolomic analysis. We are currently organizing and debating this analysis's findings.

Free Research Field

環境バイオテクノロジー

Academic Significance and Societal Importance of the Research Achievements

チョウザメのメタボローム解析は本邦初である。予備的な解析からチョウザメの代謝産物（メタボライト）に乳酸とベタインが含まれることが分かったので、本研究ではチョウザメ餌料への乳酸あるいはベタインの添加効果を見ようとしたが、このような実験も本邦初である。さらに、乳酸あるいはベタインの供給源として、試薬だけでなく、産業副産物を用いたことに社会的意義がある。具体的には、乳酸源として乳酸菌培養液の培養上清を用い、ベタイン源としてテンサイ製糖における廃糖蜜を用いた。これらバイオ・アグリ事業からの副産物を利用することで循環型社会の構築への寄与も可能となった。