2022 Fiscal Year Research-status Report
Application of body time detection by molecular timetable method for quality control of postharvest fruit and vegetables
| Project/Area Number |
21K05847
|
|---|
| Research Institution | Gifu University |
|---|
Principal Investigator |
タンマウォン マナスィカン 岐阜大学, 応用生物科学部, 准教授 (90763673)
|
|---|
| Project Period (FY) |
2021-04-01 – 2024-03-31
|
| Keywords | Circadian rhythm / Fresh produce / Quality |
|---|
| Outline of Annual Research Achievements |
Fresh produces are considered as highly perishable due to their high respiration rate and easy to deteriorate.
In this research, the effect of temperature and controlled atmosphere storage on the expression of clock genes (GmCCA1, GmLHY, GmPRR7, GmGI, GmTOC1, and GmLUX) and postharvest quality characteristics and their related genes (GmFUM1, GmCS, Gm2-OGDH, GmPPO1, GmPAL, GmDREB5) in soybean sprouts were investigated. By fitting the gene expression level obtained using the qPCR method with the cosine curve equation, it was successfully found that the circadian rhythm existed under constant dark storage conditions of soybean sprouts. A significant rhythm in clock gene expression was observed in control (20 ℃-air) soybean sprouts. In contrast, low temperature (10 ℃-air) storage diminished the cyclic expression of GmLCL1, GmPRR7, and GmTOC1, which also affected GmGI and GmLUX expression. Additionally, high CO2 (20 ℃-15% CO2 + 20% O2) concentrations during storage disturbed the circadian clock by affecting the phase and amplitude of each gene; for low O2 (20 ℃-5% O2) concentrations, it was only affected by amplitude. Interestingly, low temperature, low O2, and high CO2 maintained postharvest quality, including reduced respiration, weight loss and browning incidence. The expression behaviors of postharvest quality attribute-related genes (GmFUM1, GmCS, Gm2-OGDH, GmPPO1, GmPAL) were also influenced by the storage treatments. The findings first suggest a biologically meaningful link between clock disruption and postharvest quality maintenance of soybean sprouts.
|
| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
In the fiscal year 2022, a biologically meaningful link between clock disruption and postharvest quality maintenance of soybean sprouts was observed.
It is hoped that in future, the knowledge about the existence of the circadian clock system and its response behavior during storage will help to develop a new postharvest preservation technology by controlling circadian rhythm.
Furthermore, the results of exploring expression of clock gene in soybean sprouts were analyzed and discussed and were published in the Journal of Plant Physiology with the title ‘Existence of circadian rhythm and its response behavior under different storage conditions of soybean sprouts'.
|
| Strategy for Future Research Activity |
To investigate the effect of harvesting stress on fresh produce’s internal time, mechanical stresses (postharvest stresses) will be practically applied to the harvested soybean sprouts and quality change and clock gene expression of soybean sprouts will be analyzed during storage.
|
-
[Journal Article] Existence of circadian rhythm and its response behavior under different storage conditions of soybean sprouts2023
Author(s)
Shomodder, A., Imaizumi, T., Nagata, M., Kasai, E., Shiina, T., Tsuta, M., Thammawong, M.** and Nakano, K.*
-
Journal Title
J. Plant Physiol.
Volume: 281
Pages: 153906
DOI
Peer Reviewed
-
