2023 Fiscal Year Final Research Report
Application of body time detection by molecular timetable method for quality control of postharvest fruit and vegetables
| Project/Area Number |
21K05847
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 41040:Agricultural environmental engineering and agricultural information engineering-related
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| Research Institution | Gifu University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | Circadian rthytm / Fresh produce / Quality control |
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| Outline of Final Research Achievements |
The molecular timetable of soybean sprouts was proposed. The effect of temperature and CA storage on the clock genes and quality attributes and their related genes were examined. By fitting the gene expression level with the cosine curve equation, it was successfully found that the circadian rhythm existed under constant dark storage conditions of soybean sprouts. A significant rhythm in clock gene expression was observed in control. In contrast, low temperature storage diminished the cyclic expression of clock gene expression. Additionally, high CO2 concentrations during storage disturbed the circadian clock by affecting the phase and amplitude of each gene; for low O2, it was only affected by amplitude. Interestingly, low temperature, low O2, and high CO2 maintained postharvest quality, including reduced respiration, weight loss and browning incidence. The expression behaviors of postharvest quality attribute-related genes were also influenced by the storage treatments.
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| Free Research Field |
Postharvest Physiology
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| Academic Significance and Societal Importance of the Research Achievements |
The link between clock disruption and quality maintenance of soybean sprouts was first reported. The knowledge about the existence of the circadian clock and its response behavior during storage will help to develop a new postharvest preservation technology by controlling circadian rhythm.
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