2023 Fiscal Year Final Research Report

Remediation of the agricultural environments using the plant species with roots containing high H2O2 in the presence of woody plant organics-Fe complex via rhizosphere Fenton reaction

Research Project

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Project/Area Number	21K05869
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 41050:Environmental agriculture-related
Research Institution	Yamagata University
Principal Investigator	Wagatsuma Tadao 山形大学, 農学部, 客員教授 (70007079)
Co-Investigator(Kenkyū-buntansha)	且原真木岡山大学, 資源植物科学研究所, 教授 (00211847) 俵谷圭太郎山形大学, 農学部, 教授 (70179919) 田原恒国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員等 (70445740)
Project Period (FY)	2021-04-01 – 2024-03-31
Keywords	過酸化水素高分泌性植物 / 樹木フェノリックスー鉄錯体 / 根圏フェントン反応 / 脱窒の抑制 / OHラジカル / NO3ラジカル / Ferrihydrite
Outline of Final Research Achievements	We produced a new Fe-material complexed with wood phenolics for Fenton reaction. MB as the alternative organic pollutant was decomposed, and Pi was solubilized from rice bran and phytic acid by Fenton reaction. The highest soil H2O2 was found by the application of organic matter and amorphous Fe3+-oxide based on the newly established BES-H2O2 fluorescent method. Above portion of the correlation equation line between H2O2 concentration and pH was regarded as the H2O and O2 formation area and the below portion as the ・OH formation area by Fenton/Pseudo -Fenton reaction. Formation of H2O and O2 by chemical reaction under high pH conditions can contribute to the plant tolerant strategy for alkaline soil through the alleviation of soil aeration. NO3 radical was suggested to be formed in the system composed of Fe, H2O2 and nitrate, and to be combined with the soil organic matters. The highest root apoplast H2O2 in Asteraceae plants could be scavenged by the H2O2 aquaporin inhibitor AgNO3.
Free Research Field	植物栄養学・土壌学
Academic Significance and Societal Importance of the Research Achievements	①間伐材に大量に含まれ難生物分解性phenolicsをFeと錯体化し資源有効化を図り、以下を初めて明らかにした。②有機体PからのPi遊離がFenton純化学反応で可能。③植物・土壌のH2O2の濃度を、簡易なBES-H2O2蛍光顕微鏡法で測定。④有機物と非晶質Fe3+酸化物の投入で、土壌H2O2濃度を上昇。⑤Fenton系でのNO3-の存在でNO3ラジカルが生成し、これは土壌有機物に取り込まれ、容易に脱窒を回避できる。⑥アルカリ性で土壌H2O2が化学的にH2OとO2に分解され、通気性改善で植物のアルカリ土壌耐性に貢献。⑥キク科植物根apoplastの高H2O2とaquaporinとの関連性。