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2023 Fiscal Year Final Research Report

Elucidation of in situ lignin biodegradation using bacterial sensor

Research Project

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Project/Area Number 21K05872
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 41050:Environmental agriculture-related
Research InstitutionNagaoka University of Technology

Principal Investigator

Kamimura Naofumi  長岡技術科学大学, 工学研究科, 准教授 (50646528)

Co-Investigator(Kenkyū-buntansha) 亀井 一郎  宮崎大学, 農学部, 教授 (90526526)
Project Period (FY) 2021-04-01 – 2024-03-31
Keywordsリグニン / センサー / フェルラ酸 / バニリン酸 / 環境分析 / 転写制御 / 蛍光タンパク質
Outline of Final Research Achievements

This study aims to clarify the biodegradation of lignin by fungi and bacteria in nature. Bacterial sensors detecting lignin-derived compounds were used to investigate the presence of lignin-derived compounds in the environment and whether bacteria actually metabolize lignin degradation products produced by fungal wood decay. Analysis of environmental samples suggested that the bacteria metabolize a wide variety and low concentrations of aromatic compounds (monomers and oligomers) that could not be identified by chemical analysis. Analysis of decayed wood showed that decay by white rot fungus Phanerochaete chrysosporium enhanced the sensor response. This result demonstrated that bacteria actually metabolize the aromatic compounds produced by fungal lignin degradation, and provided an evidence of bacterial metabolism of lignin biodegradation products in nature.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

自然界におけるリグニンの分解においてブラックボックスであった、真菌による高分子リグニンの分解と細菌によるリグニン由来低分子芳香族化合物の代謝をつなぐ知見を得るためのツールとして、細菌センサーが有効であることを実証し、リグニン由来化合物のモデル分解細菌として位置づけられているSphingobium lignivorans SYK-6株が、環境中または白色腐朽菌によるリグニンの生分解生成物を代謝できることを明らかにした。また、本研究では、センサー細菌に関する派生テーマとして、リグニンの化学分解物からポリマー原料を生産する細菌の改良に有益な、ポリマー原料化合物の応答センサーを開発した。

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Published: 2025-01-30  

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