Analysis of genetically modified mice to elucidate the function of the ESCRT complex during the cleavage stage.

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K05890
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42010:Animal production science-related
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: Takabayashi Shuji 浜松医科大学, 光医学総合研究所, 准教授 (70372521)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: ESCRT-III / Chmp2 / Chmp3 / Chmp4 / i-GONAD

Outline of Final Research Achievements

The Chmp2a gene, a subunit of ESCRT-III, was discovered as the causative gene for a novel mutant mice that spontaneously develops amyotrophic lateral sclerosis (ALS)-like pathology. In this study, we generated KO mice for the Chmp family gene, a component protein of ESCRT-III that functions in cell division and membrane remodeling. Phenotypic analysis of these KO mice during early development at the cleavage stage was performed to clarify the function of ESCRT-III during the cleavage stage of fertilized eggs.

Free Research Field

発生学

Academic Significance and Societal Importance of the Research Achievements

ESCRT-IIIはMVB形成や細胞質分裂等の膜のリモデリングに関係している。細胞などを用いた研究で機能解析が行われてきたが、マウスを用いた解析がほとんどされていなかった。本研究において、ESCRT-IIIの構成成分であるChmpファミリー遺伝子のKOマウスを作製に成功した。卵割期自体には影響がないことが分かったので、今後さらなる解析が期待される。
KOマウスの作製はi-GONAD法を用いて行った。そのために効率的なi-GONAD法の開発研究を行った。