Elucidation of specific oligosaccharide metabolism for establishment of silage preparation

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K05898
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42010:Animal production science-related
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Nixhimoto Mamoru 国立研究開発法人農業・食品産業技術総合研究機構, 食品研究部門, 上級研究員 (30399381)
• Co-Investigator(Kenkyū-buntansha): 小林 寿美 国立研究開発法人農業・食品産業技術総合研究機構, 畜産研究部門, 主任研究員 (80609701)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: Loliose / サイレージ

Outline of Final Research Achievements

This study was conducted to evaluate the functionality of loliose, especially its utilization by microorganisms related to silage. As a result, loliose-utilizing microorganisms were identified by culture tests using prepared loliose, and candidate genes were identified from the genome information of the reference strain. In addition, analysis of culture supernatants suggested that there are at least three patterns of loliose utilization. Furthermore, we succeeded in selecting more than 20 strains of silage-associated spore-forming bacteria by integrated culture test using loliose-enriched solution. No silage deterioration due to the addition of loliose-enriched solution in the silage conditioning test was observed.

Free Research Field

糖質科学

Academic Significance and Societal Importance of the Research Achievements

本研究によるロリオース分解酵素の同定は酵素科学分野における新たな知見として学術的価値が高い。また、ロリオースを資化できる微生物の単離もサイレージ発酵中のロリオースの影響を評価する上で貴重なデータである。さらに、これらのロリオースに関するデータの蓄積は、新たな機能性オリゴ糖の実用的開発を加速するものである。