2023 Fiscal Year Final Research Report
Chrono-nutrition of the pig—manipulation of the time enhancing membrane digestive enzymes activities with time-restricted feeding
Project/Area Number |
21K05910
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 42010:Animal production science-related
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Research Institution | Azabu University |
Principal Investigator |
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Project Period (FY) |
2021-04-01 – 2024-03-31
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Keywords | ブタ / 時間栄養学 / 時計遺伝子 / グルコーストランスポーター / 腸内細菌叢 |
Outline of Final Research Achievements |
In this study, time-restricting feeding was implemented to isolate the effects of lighting environment and feed intake on mRNA abundances of clock genes in the suprachiasmatic nucleus and peripheral tissues, mRNA abundances of glucose transporters in the jejunum, and bacterial flora in the cecum. Diurnal variation was observed in the abundances of mRNA of several clock genes in the suprachiasmatic nucleus and peripheral tissues and in the glucose transporter in the jejunum. The abundances of these genes differed by approximately 12 hours when dark period-limited feeding was compared with light period-limited feeding. This suggests that feed intake has a strong influence on the diurnal variation of mRNA abundances of clock genes and glucose transporters in the suprachiasmatic nucleus and peripheral tissues in pigs. Diurnal variation at the species level was also observed in the gut microbiota.
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Free Research Field |
家畜栄養
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Academic Significance and Societal Importance of the Research Achievements |
離乳育成豚を24時間不断給餌の条件で飼育すると、空腸の時計遺伝子とグルコーストランスポーターのmRNA発現量が日内変動した。この実験では明期の飼料摂取量が高かったので、これらの遺伝子発現の日内変動が、光環境あるいは飼料摂取、どちらの影響を受けたのか明確にできなかった。本研究では、明期限定給餌と暗期限定給餌で、それぞれの遺伝子発現の日内変動に12時間のずれがあり、とくに末梢組織では飼料摂取の影響を強く受けることを明らかにできた。また、腸内細菌叢にも飼料摂取による日内変動を観察した。これは、腸内細菌叢の代謝産物にも日内変動があり、ひいては、宿主ブタへの影響にも日内変動があることを示唆している。
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