2023 Fiscal Year Final Research Report
Growth mechanism in animal eye development regulated by placental C3 and analysis of placenta derived factors for precocial neonates
| Project/Area Number |
21K05936
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 42020:Veterinary medical science-related
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 網膜 / 補体 / ミクログリア / 胎盤 / ウマ / 遺伝子 |
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| Outline of Final Research Achievements |
In chickens, the complement protein C3a and its receptor C3aR are involved in eye development. In mammals, the placenta is an abundant organ for C3 fragment proteins. In the present study, both C3a and C3aR were examined in the early forming mouse retina, but both expressions were low, and C3 fragment proteins were not distributed inside the retina.
The association of the complement receptor CR3 with microglia in the retina of mouse neonates was examined; postnatal changes in the expression of CR3 and the microglial marker Iba1, as well as its intraretinal localization, were not consistent.
Expressed genes in the horse placentas and transition into blood were examined; ANXA2 and IT2MB were detected at high frequencies, but not specifically in gestational serum and cord blood.
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| Free Research Field |
新生子の生物学
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| Academic Significance and Societal Importance of the Research Achievements |
補体C3aと受容体C3aRはマウスの眼球形成に関与しないことが分かった。また、新生子の網膜におけるミクログリアの制御に、CR3は関与しないと考えられた。一方で、妊娠期の母体において、とくに乳腺で補体C3のフラグメントタンパクが作られていることが分かった。このことは泌乳によって新生子に補体タンパクが移行し、生理学的な役割を持つ可能性がある。
ウマ胎盤で特異的に作られ、母体血や臍帯血に移行する因子を見つけることは出来なかった。しかし、ウマ胎盤で高発現している遺伝子リストを作成することが出来、このことはウマの妊娠生理や妊娠のモニタリングを行うための重要な研究材料になり得る。
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