Study of the splicing regulation in response to heat stress.

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K06018
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 43010:Molecular biology-related
• Research Institution: Kobe University
• Principal Investigator: INOUE KUNIO 神戸大学, 理学研究科, 教授 (40252415)
• Co-Investigator(Kenkyū-buntansha): 松花 沙織 神戸大学, 理学研究科, 講師 (70767251)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 遺伝子発現 / 熱ストレス / 選択的スプライシング

Outline of Final Research Achievements

It has been shown that alternative splicing of various genes is regulated in response to heat stress in mammalian cells. Our previous study identified RNA-binding proteins required for the splicing regulation of human hsp105 and tnrc6a genes upon heat stress. However, it has been remained to fully solve the mechanism how the heat stress-responsive alternative splicing is regulated by the RNA-binding proteins such as hnRNPK and SRSF proteins. In the present study, we have identified the amino acid motif of the SRSF proteins, necessary for inducing the heat stress-responsive splicing in mammalian cells. In addition, we are constructing a reporter system to visualize the heat stress-responsive alternative splicing with high sensitivity in zebrafish.

Free Research Field

分子生物学、発生生物学

Academic Significance and Societal Importance of the Research Achievements

細胞は各種ストレスを含む細胞環境に応答して遺伝子発現制御を行っている。本研究では温度に依存した選択的スプライシングの誘導機構に関する新たな知見や個体レベルでの理解に結びつく成果をあげつつあり、今後、さまざまな細胞ストレス応答や細胞死における選択的スプライシングを介した遺伝子発現制御機構や、がん化・疾患における異常スプライシング機構の理解にも結びつくものと期待される。