2023 Fiscal Year Final Research Report
Energy conversion from ion motive force to torque in the flagellar motor
| Project/Area Number |
21K06073
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 43030:Functional biochemistry-related
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| Research Institution | Chuo University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 生体分子モーター / 一分子計測 / イオンチャネル |
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| Outline of Final Research Achievements |
Bacterial flagellar motors convert the electrochemical potential formed by the intracellular and extracellular concentration differences of protons or sodium ions and the membrane potential into the torque. The motor consists of a stator and a rotor, and the ion-influx through the channels of the stator complex causes an interaction between them, generating the torque. A stepwise displacement is detected in the motor rotating, which is thought to reflect the interaction processing between the stator and the rotor. Therefore, we analyzed the steps of a slowly rotating motor under physiological conditions to investigate the relationship between the ion flow and the torque generation. As a result, differences in the effects of ion concentration differences and membrane potential on the motor were observed.
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| Free Research Field |
生物物理
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| Academic Significance and Societal Importance of the Research Achievements |
固定子がどのようにイオンの流れを回転力に変換しているのか不明である。本研究は、生理条件下で「イオンの流れをトルクに変換する」一連の反応サイクルが遅い固定子によって駆動するモーターの入出力を解析することで、べん毛モーターのエネルギー変換メカニズムを明らかにする手がかりとなる。さらに、これらの情報はべん毛モーター研究だけでなく、生物分子モーターの動作原理の解明の一端を担う重要な研究課題として位置づけられる。そして、それらの知見は人工的な分子マシンを構築する際に多くの知見をもたらすと期待される。
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