2023 Fiscal Year Final Research Report
Creation of molecules regulating the self-incompatibility in Brassicaceae plants based on the protein structures.
| Project/Area Number |
21K06110
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 43040:Biophysics-related
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
MORIWAKI Yoshitaka 東京大学, 大学院農学生命科学研究科(農学部), 助教 (70751303)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
村瀬 浩司 東京大学, 大学院農学生命科学研究科(農学部), 特任准教授 (50467693)
|
|---|
| Project Period (FY) |
2021-04-01 – 2024-03-31
|
| Keywords | 農芸化学 / タンパク質科学 / タンパク質構造予測 / 自家不和合性 |
|---|
| Outline of Final Research Achievements |
In this study, we showed that the tertiary structure of SRK and SP11, the key proteins that regulate self-incompatibility in Brassicaceae, could be modeled with very high confidence using AlphaFold2 (ColabFold) and their multiple sequece alignment (MSA). Modeling of their structures and complexes has been considered challenging in the field of protein structure prediction because of their exceptional number of disulphide bonds and sequence diversity. These results are expected not only to simplify non-experimental prediction of naturally occurring SRK-SP11 protein complexes, but also to enable their artificial creation.
|
| Free Research Field |
生物物理学
|
| Academic Significance and Societal Importance of the Research Achievements |
SRK, SP11タンパク質構造およびそれらの複合体の高精度な予測が可能になったことで、アブラナ科の自家不和合性機構が構造的に可視化され、分子論的な理解が大きく進むことが期待される。また、この研究成果を元に将来的に人工的なSP11分子の創出を行うことでアブラナ植物の受粉機構を人為的に制御することが可能になれば、我々人類の食卓に上る、キャベツ、ブロッコリー、小松菜などの主要なアブラナ科の野菜を生産する育種現場において、遺伝的に望ましい形質を持つ品種を選択的に保存または改変することができるようになることが期待される。
|
