2021 Fiscal Year Annual Research Report
Nanoscale probing and visualization of Annexin A5 assembly, function, and dynamics on native cellular membranes
| Project/Area Number |
21K06111
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| Research Institution | Kanazawa University |
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Principal Investigator |
Marchesi Arin 金沢大学, ナノ生命科学研究所, 特任助教 (80882240)
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| Project Period (FY) |
2021-04-01 – 2022-03-31
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| Keywords | annexins / atomic force microscopy / membrane proteins |
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| Outline of Annual Research Achievements |
The aim of this project was to understand how different biophysical and biochemical parameters regulate annexin A5 supramolecular assembly and ultimately visualize annexin arrays on native membranes from isolated cells. We obtained preliminary data demonstrating that membrane fluidity is a key driver regulating annexin polycrystalline lattice formation and crystallographic configuration. The inital experimental observations were carried out on model membranes (planar artificial bilayer)by means of fluorescence imaging and high-speed atomic force microscopy. Photoswitchable compounds were also used to manipulate membrane properties and demonstrate annexin supramolecular polymorphism.
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