2023 Fiscal Year Annual Research Report
哺乳類のDNA複製タイミング制御におけるゲノムとエピゲノムの機能的リンク
| Project/Area Number |
21K06129
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
SHARIF JAFAR 国立研究開発法人理化学研究所, 生命医科学研究センター, 専任研究員 (00577968)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 3D genome / Epigenome / Replication timing |
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| Outline of Annual Research Achievements |
The higher order mammalian genome is organized into euchromatin associated A, and heterochromatin associated B, compartments. In this project, I analyzed the mechanistic link between A/B compartments and early/late replication timing. By taking advantage of degron-based acute depletion of transcriptional machinery, I have found that transcriptional elongation functions as a molecular link to connect A/B compartments and early/late replication timing. Furthermore, by taking advantage of a conditional gene knockout strategy, I have been able to identify an epigenetic mechanism that functions directly downstream of transcriptional elongation to connect compartmentalization with replication timing.
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