Organellar segregation in yeast sporulation.

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K06145
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 44010:Cell biology-related
• Research Institution: University of Tsukuba
• Principal Investigator: Suda Yasuyuki 筑波大学, 医学医療系, 助教 (10553844)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 生体膜 / 膜輸送 / 出芽酵母 / 胞子形成 / 減数分裂

Outline of Final Research Achievements

The aim of this study was to elucidate the molecular mechanisms governing the morphological changes of the functional domain of the endoplasmic reticulum (ER), ERES and their distribution to spores during sporulation in budding yeast, and their contribution to the formation of the prospore membrane (PSM) formation. First, the morphology and distribution of the ER and ERES during meiosis and sporulation in wild-type were analyed, and screening of the mutants showing defects in the distribution of the ERES was conducted. The phenotype was analysed in detail by live imaging. The results showed a transient arrest of the secretory pathway with a reduction of ERES during meiosis. The membrane traffic-associated organelle disappeared from the cell during meiosis, triggering PP1-Gip1 to re-form inside the PSMs upon the onset of their formation.

Free Research Field

細胞生物学

Academic Significance and Societal Importance of the Research Achievements

真核生物には老化に伴う個体の寿命が存在するが、配偶子形成を経た世代交代により個体寿命・老化はリセットされる。この現象は胞子形成を経た出芽酵母においても認められている。現在までに、出芽酵母の減数分裂・胞子形成時のオルガネラ分配や前胞子膜は、個体寿命・老化のリセット、すなわち若返りメカニズムへの関与が示唆されている。しかしながらその詳細なメカニズムについては明らかになっていない。本研究により前胞子膜形成のメカニズムの一端が明らかとなった。今後は、取得した変異株における個体寿命リセットなど詳細な解析を通じ、個体寿命・老化との関連などが少しずつ明らかになっていくと期待される。