2023 Fiscal Year Final Research Report
Multi-functionalization of the NonoLuc-based HiBiT tag
| Project/Area Number |
21K06202
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44020:Developmental biology-related
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| Research Institution | Kochi University of Technology |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | タギング |
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| Outline of Final Research Achievements |
Establishing knock-in animals using genome editing technology is a time-consuming process. Therefore, if a single peptide tag can be multi-functionalized, it will accelerate research. This study aimed to multi-functionalize the HiBiT luminescent tag derived from NanoLuc. The HiBiT tag reconstitutes NanoLuc by binding to LgBiT with high affinity. Thus, the HiBiT tag has the potential to be used as an affinity tag. In fact, we demonstrate that the HiBiT tag can be used for protein knockdown in combination with an F-box protein, and for visualization of proteins within cells in combination with fluorescent proteins.
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| Free Research Field |
分子発生生物学
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| Academic Significance and Societal Importance of the Research Achievements |
タンパク質に機能的なタグを付加することは、タンパク質の検出や分析を容易にし、研究を促進させる。一方、タグの導入を内在遺伝子に対して行う場合は、時間とコストが問題になる。したがって、1つのタグを多機能化することは、タンパク質の解析の迅速化と高度化に貢献することが期待される。本研究では、HiBiTタグが発光タグばかりでなく、アフィニティタグとしての利用可能なことを示し、HiBiTタグの利便性を格段に高めることができる可能性を示した。
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