2023 Fiscal Year Final Research Report
Verification of mutual regulation hypothesis for electron flow and light harvesting system in photosynthesis
| Project/Area Number |
21K06217
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44030:Plant molecular biology and physiology-related
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| Research Institution | Okayama University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | Photosynthesis / Plant physiology / biochemistry / Photosynthetic control / cytochrome b6f |
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| Outline of Final Research Achievements |
The photosynthetic electron transfer system is protected by NPQ, cyclic electron transfer, and photosynthetic control (PCON), while the light-harvesting antenna complex (LHC) optimizes light energy. This study generated and characterized the PCON mutant. The data showed that PCON is driven by acidification of the thylakoid lumen and is important for photosynthetic growth under high light. In addition, mass spectrometry-based interactome analysis proposed a structural role for outer LHCI under high light where NPQ is inducible, supported by biochemical experiments using an outer LHCI subunit-deficient mutant.
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| Free Research Field |
植物生理学
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| Academic Significance and Societal Importance of the Research Achievements |
Photosynthetic controlはチラコイド膜ルーメンの酸性化に応答したb6fの電子伝達速度調整であり光合成的細胞成長にも影響が大きいことを見いだした。この機能を発揮するb6fの構造的な基盤が明らかになればさらなる機能解明が期待される。また、強光下でLHCがはたす構造的な役割も一端が見え始めてきた。解析対象を広げることで、LHCの機能をより動的に捉えることができると期待される。
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