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2023 Fiscal Year Final Research Report

Research on chloroplast-associated protein, SLH conserved in land plants

Research Project

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Project/Area Number 21K06251
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44040:Morphology and anatomical structure-related
Research InstitutionKumamoto University

Principal Investigator

Takechi Katsuaki  熊本大学, 大学院先端科学研究部(理), 准教授 (70515501)

Co-Investigator(Kenkyū-buntansha) 高野 博嘉  熊本大学, 大学院先端科学研究部(理), 教授 (70242104)
Project Period (FY) 2021-04-01 – 2024-03-31
Keywords葉緑体 / 葉緑体内構造 / オルガネラ / 植物進化
Outline of Final Research Achievements

Analysis of the localization of PpSLHs proteins in Physcomitrella patens revealed that they were aggregated around the envelope of chloroplasts, and were also localized in the giant envelope membrane, which is a single membrane in the PpSLH quadruple gene disruptant. Examination of the effect of each domain on chloroplast localization revealed that the presence of the C-terminal and transmembrane domains is necessary for normal localization, and that the C-terminal domain is predicted to interact with unknown chloroplast inter-envelope proteins, resulting in dot-like localization. No interaction was found between the SLH domain and PG. PpSLHs are predicted to be embedded in the chloroplast inner envelope membrane, interact to PG between the domains other than the SLH domain and the envelope membrane, and are involved in the stability of the inner envelope membrane.

Free Research Field

植物形態学

Academic Significance and Societal Importance of the Research Achievements

本研究では,葉緑体の進化過程において,共生菌がもっていたPG層の進化も重要なファクターではないかと考えた。細菌がもつPG関連タンパク質の多くは,植物には保存されていないが, SLHドメインをもつタンパク質は,陸上植物に広く保存されている。葉緑体の進化において,SLHタンパク質が重要な働きを持っているのではないかと考え,PGを持つヒメツリガネゴケにおいて機能解析を行った。葉緑体内に巨大小胞が形成される変異体はこれまでに例がなく,この変異体の解析を通じて,葉緑体包膜の動態を維持する未知のシステムの存在が明らかになるだけでなく,葉緑体の進化に至った要因を見いだすことができると考えている。

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Published: 2025-01-30  

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