Establishment of a Comprehensive Lipid Quantification Method Using Double Standard Lipidome Analysis

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K06512
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
• Research Institution: National Institutes for Quantum Science and Technology (2023); Kyushu University (2021-2022)
• Principal Investigator: Nakao Motonao 国立研究開発法人量子科学技術研究開発機構, 量子生命科学研究所, 主任技術員 (60457306)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: lipidomics

Outline of Final Research Achievements

Nonalcoholic steatohepatitis (NASH) is characterized by inflammation associated with fatty liver and progresses to irreversible fibrosis as the disease advances. Furthermore, liver fibrosis can progress to cirrhosis and liver cancer, making it essential to develop diagnostic techniques that can clearly distinguish between fatty liver and NASH at an early stage. Metabolome analysis is a simple and useful technique for identifying diseases through in vitro diagnostics; however, mass spectrometry (MS) cannot perform absolute quantification. We report here the development of a method for calculating quantitative values for lipids, which often have many positional isomers, to aid in clinical diagnosis.

Free Research Field

情報科学

Academic Significance and Societal Importance of the Research Achievements

メタボローム解析は体外診断で疾患を同定できる有用な技術ですが、測定に使用する質量分析装置は絶対定量ができない。特に位置異性体が多い脂質は、その標準品を用意することすら難しいため、相対定量も困難な状況である。そこで、標準品の存在しない脂質の定量方法を開発すると同時に、未だに体外診断のできないNASHの診断方法に寄与できるのではないかと考えた。メタボローム定量は、今後すべての診断や創薬においてエビデンスを提供する重要な技術に発展します。