Identification of redox partner for a novel arginine hydroxylase and establishment of high level production system for anti-tubercular agent

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K06629
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 47050:Environmental and natural pharmaceutical resources-related
• Research Institution: Hiroshima University
• Principal Investigator: Kumagai Takanori 広島大学, 医系科学研究科(薬), 准教授 (70274058)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: アルギニン水酸化酵素 / 電子伝達系 / 抗癌剤 / ヒドロキシウレア / 抗結核薬 / D-サイクロセリン / 異種生産

Outline of Final Research Achievements

In the present study, in order to identify the redox partner for a novel arginine hydroxylase, DcsA, three ferredoxins (FDXs) and two FDX reductases (FDRs) from a D-CS-producing microorganism were obtained. However, the redox partner for DcsA could not be determined. Considering putidaredoxin (PDX) and PDX reductase (PDR) from Pseudomonas putida as the redox partner for DcsA, expression system for each protein was constructed using Escherichia coli as a host, and each protein was expressed as soluble protein.

Free Research Field

微生物薬品学

Academic Significance and Societal Importance of the Research Achievements

本研究で得られた成果は, 大腸菌を宿主とした抗癌剤ヒドロキシウレア（HU）の生産系の構築につながる可能性があり, 学術的に意義があると思われる。また, HU生産系の構築が達成されれば, D-CSの大腸菌を宿主とした実用レベル高生産系が確立され, より安全で安価なD-CSの供給につながる可能性があり, 社会的に意義があると考えられる。