Development of the useful substance production system for genus Dendrobium using mycorrhizal fungi.

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K06635
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 47050:Environmental and natural pharmaceutical resources-related
• Research Institution: Nihon University
• Principal Investigator: TAKAMIYA Tomoko 日本大学, 薬学部, 講師 (50513917)
• Co-Investigator(Kenkyū-buntansha): 遊川 知久 独立行政法人国立科学博物館, 植物研究部, グループ長 (50280524) ; 辻田 有紀 佐賀大学, 農学部, 准教授 (80522523)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 共生菌 / ラン科植物 / ビベンジル合成酵素 / 物質生産

Outline of Final Research Achievements

Dendrobium officinale Kimura & Migo is an important medicinal plant in orchid that produces various bibenzyl and phenanthrene derivatives. Bibenzyl biosynthesis is regulated by bibenzyl synthase (BBS). Although six genes of the BBS family have been registered from D. officinale, their gene regulation mechanisms remain unclear. The infection of orchid with mycorrhizal fungi reportedly increases the expression of genes involved in biosynthesis. However, the effect of mycorrhizal fungi on bibenzyl biosynthesis is unknown. In this study, we investigated the effects of three mycorrhizal fungi isolated from D. officinale in Japan on BBS gene expression and bibenzyl production over time. One of the Tulasnellaceae operational taxonomic units induced BBS gene expression and increased two bibenzyls, gigantol and dendrophenol at specific time points. These results suggest that it is possible to optimize metabolite production by selecting suitable symbiotic fungi.

Free Research Field

植物の二次代謝産物と生合成遺伝子

Academic Significance and Societal Importance of the Research Achievements

本研究では、共生菌と植物の共生関係に注目し、菌が植物の生合成遺伝子の発現に及ぼす影響を調べた。その結果、特定の菌がビベンジル合成に関与する遺伝子の発現に影響を与えることが明らかになった。さらに、共生菌との共培養によって代謝物量が増加していた。今回の結果から、適切な共生菌を選択することによって目的の代謝物生産を最適化できる可能性が示唆された。