2023 Fiscal Year Final Research Report
Establishment of efficacy index and pharmacokinetic analysis of antiplatelet drugs based on proteomic analysis
| Project/Area Number |
21K06709
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 47060:Clinical pharmacy-related
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| Research Institution | Gunma University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
荒木 拓也 群馬大学, 大学院医学系研究科, 准教授 (00568248)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 血小板 / 薬動力学 |
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| Outline of Final Research Achievements |
In this study, we will conduct proteome analysis after ADP stimulation of platelet-rich plasma with altered platelet aggregation ability, and search for proteins that quantitatively reflect platelet aggregation ability. PRP is prepared by centrifugation using fresh blood obtained from a healthy person, and immediately after activating platelets by adding ADP, it is subjected to reductive alkylation treatment, and the protein is fragmented with trypsin. We established LC-TOF-MS conditions that allow detection of amino acid sequences specific to the process in which Calpain cleaves. We are continuing similar studies using rat samples for the purpose of quantitative analysis using animals.
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| Free Research Field |
医療薬学
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| Academic Significance and Societal Importance of the Research Achievements |
血小板凝集能の保持時間が短く、採血後速やかに測定を完了させる必要があるため、抗血小板薬投与時に血小板凝集抑制作用の指標を臨床で継続的に監視することはほとんどない。さらに、抗血小板薬は血小板凝集カスケードの様々な過程に影響を及ぼしており、血中薬物濃度推移と血小板凝集能のプロファイルは一致せず、血中薬物濃度をそのまま血小板凝集能の指標とすることもできない。
本研究により、抗血小板作用を物質レベルで評価する手法の確立の手がかりが得られたことにより、血中薬物濃度と同時に測定することで、汎用性の高い生理学的薬動力学モデルを構築できる。
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