2023 Fiscal Year Final Research Report
Molecular regulation of cell adhesion machinery by epidermolysis bullosa-associated protein Exophilin-5
| Project/Area Number |
21K06866
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 49010:Pathological biochemistry-related
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 表皮水疱症 / Exophilin-5 / Rab27 / 調節性分泌 / 細胞接着 |
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| Outline of Final Research Achievements |
To investigate the role of Exophilin-5 in epidermal cells and its molecular mechanism, we have searched for specific binding proteins of Exophilin-5 and several proteins involved in cell adhesion were identified. Analysis of these novel binding proteins revealed that Exophilin-5 functions not only in hemi-desmosome formation but also in the formation of other adhesion machinery. Furthermore, deletion of Exophilin-5 inhibited hemi-desmosome and other adhesion machinery formation and reduced cell adhesion ability. We examined wound healing assay in knockout mouse skin and the wound healing of knockout mice was delayed compared with wild-type mice.
These results indicate that Exophilin-5 plays an important role in the homeostasis of the cell adhesion machinery.
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| Free Research Field |
細胞生物学
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| Academic Significance and Societal Importance of the Research Achievements |
表皮水疱症は、重篤な先天性疾患であり、日常生活が著しく制限され、未だに対症療法しかない難病である。Exophilin-5は単純型表皮水疱症の原因遺伝子として同定されたが、その詳細な作用機序はほとんどわかっていなかった。本研究により、Exophilin-5が複数の細胞接着装置構成たんぱく質と相互作用し、細胞接着装置の恒常性維持において重要な役割を持っていることが示されたことにより、単純型表皮水疱症の治療において重要な知見を得ることができた。
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