2021 Fiscal Year Annual Research Report
Investigation of alpha-synuclein associated synaptic dysfunction by unraveling the molecular mechanisms using 3D brain organoid model
| Project/Area Number |
21K06896
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
Jo Junghyun 沖縄科学技術大学院大学, 行動の脳機構ユニット, スタッフサイエンティスト (50861126)
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| Co-Investigator(Kenkyū-buntansha) |
TAOUFIQ Zacharie 沖縄科学技術大学院大学, 細胞分子シナプス機能ユニット, スタッフサイエンティスト (10549348)
Arbuthnott Gordon 沖縄科学技術大学院大学, 行動の脳機構ユニット, 教授 (20455570)
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| Project Period (FY) |
2021-04-01 – 2022-03-31
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| Keywords | Parkinson's disease / Embryonic stem cell / Organoid / SNCA / GBA1 |
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| Outline of Annual Research Achievements |
As a Principal Investigator, Dr. Junghyun Jo decided to discontinue the KAKENHI project in early January 2022 because he resigned from his position at OIST, there were not much research achievements for about 10 months. Following the Specific Aim 1 for Year 1 and 2, he has successfully created hMLO-hSLO fusion organoid using wildtype and GBA1 human embryonic stem cells that showed reciprocal nigro-striatal and striato-nigral projections. Using the fusion organoid, he has validated the formation of synapses between dopaminergic neurons and GABAergic neurons at each area. Also, he confirmed the formation of alpha-synuclein aggregation in the fusion organoids.
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