Development of novel staining method to enable 3D pathological diagnosis.

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K06903
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 49020:Human pathology-related
• Research Institution: Osaka University
• Principal Investigator: Nojima Satoshi 大阪大学, 大学院医学系研究科, 准教授 (40528791)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: ３次元イメージング / 組織透明化 / 臨床病理診断 / 炎症性腸疾患 / 潰瘍性大腸炎

Outline of Final Research Achievements

Although the utility of tissue clearing and three-dimensional imaging techniques in clinical histopathology has been established, further methodological developments are required for their use in clinical practice. We developed a novel periodic acid-FAM hydrazide staining method (PAFhy stain), which is based on a principle similar to the classical PAS stain. This method could be applied to 3D imaging with a tissue-clearing technique; it enabled clear and detailed imaging of the 3D architecture of crypts in human colorectal mucosae. The 3D imaging also revealed the 3D distributions of neutrophils in cryptitis and crypt abscesses in ulcerative colitis tissues. Quantitative analysis of crypts based on 3D morphological changes enabled differential diagnosis among ulcerative colitis, Crohn's disease, and non-inflammatory bowel disease colitis. Therefore, PAFhy staining and the 3D imaging with it are promising approaches for next-generation experimental and clinical histopathology.

Free Research Field

病理学

Academic Significance and Societal Importance of the Research Achievements

本研究で新たに開発されたPAFhy stain法は、3次元病理組織学における特殊染色に位置する有用な手法であり、基礎病理学・臨床病理学の新たな基盤的技術となりえるものである。これらがより標準化され他の技術と組み合わされることで新たなプラットフォームが確立できる可能性があり、これを元に疾患に根差した多くの病理学的研究、臨床的研究が蓄積されることで、新たな診断基準・臨床予測法の確立、新規治療法の開発に繋がることが期待できる。