2023 Fiscal Year Final Research Report
Molecular basis for determining degradation and recycling of EGFR on endosomes
| Project/Area Number |
21K07104
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 50010:Tumor biology-related
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | EGFR / メンブレントラフィック / クラスリンアダプター / エンドソーム |
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| Outline of Final Research Achievements |
Endocytic pathway of EGFR has been intensively studied. However, recycling pathway of EGFR remains poorly understood. In the present study, I found the role of clathrin adaptors AP-1 and GGA2 in EGFR recycling. They recognize EGFR on the recycling endosomes which is positive for Rab11, and knockdown of each adaptor decreases the recycling rates of EGFR. I also observed the upregulations of AP-1 and GGA2 on endosomal structures in tumors of some cancer tissues, which suggests the importance of AP-1 and GGA2 in growth and invasion of cancer cells.
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| Free Research Field |
腫瘍生物学
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| Academic Significance and Societal Importance of the Research Achievements |
学術的意義：EGFRのリサイクリングの分子基盤はよくわかっていなかったが、本研究によりクラスリンアダプターのAP-1およびGGA2の関与を明らかにした。
社会的意義：分子標的薬によるEGFRの阻害は癌治療の戦略であるが、その使用は数年後に薬剤耐性細胞を伴う。本研究で見出したEGFR阻害は新たな癌治療戦略であり、新たな抗癌剤による癌治療の可能性が示唆された。
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