• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2023 Fiscal Year Final Research Report

Development of a human coagulation factor assay using hemophilia A porcine plasma

Research Project

  • PDF
Project/Area Number 21K07324
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 52010:General internal medicine-related
Research InstitutionJichi Medical University

Principal Investigator

Kashiwakura Yuji  自治医科大学, 医学部, 講師 (40382890)

Co-Investigator(Kenkyū-buntansha) 大森 司  自治医科大学, 医学部, 教授 (70382843)
Project Period (FY) 2021-04-01 – 2024-03-31
Keywords第VIII因子 / 血友病A
Outline of Final Research Achievements

The aim of this study is to establish a versatile FVIII assay for measurement in all hemophilia A patients using hemophilia A porcine plasma and porcine FVIII-deficient plasma, using a coagulation one-step method based on activated partial thromboplastin time. The production of hemophilia A pigs was investigated, but no litters were obtained. Some monoclonal antibodies capable of removing porcine FVIII from wild-type porcine plasma and a ligand that binds to porcine VWF were obtained. Porcine FVIII-deficient plasma prepared using these antibodies will enable the establishment of a highly versatile FVIII assay system.

Free Research Field

血液凝固学

Academic Significance and Societal Importance of the Research Achievements

本研究における測定法が可能になることで、さまざまな血友病A患者における急性出血や周術期出血に対し、適切な治療が可能となる。また、多くの医療機関が採用する現行の検査法に用いる測定機器を利用し、周術期の迅速な測定が可能となる。さらに、血友病A患者のインヒビター測定にも利用が可能であり、すべての血友病A患者の測定を可能とする汎用性の高いFVIII測定系が確立できる。また本研究は、遺伝子改変動物試料を用いた臨床検査薬開発の先駆的研究であり、遺伝子改変動物試料を利用した新しい開発研究分野の創出にもなる。

URL: 

Published: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi