2023 Fiscal Year Final Research Report
Molecular pathogenesis of psychosocial stress-related asthma using immune humanized mice
| Project/Area Number |
21K07400
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 52010:General internal medicine-related
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
伊藤 亮治 公益財団法人実験動物中央研究所, 実験動物応用研究部, 室長 (60425436)
岡山 吉道 日本大学, 医学部, 兼任講師 (80292605)
権 寧博 日本大学, 医学部, 教授 (80339316)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 免疫ヒト化マウス / ストレス / 喘息 |
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| Outline of Final Research Achievements |
In this study, we investigated the molecular pathogenesis of stress-related asthma in wild-type and immune-humanized mice. In wild-type mice, restraint stress following antigen exposure induced eosinophilic airway inflammation. Global gene expression analysis of lung tissue identified increased expression of a group of genes reported to be involved in asthma pathogenesis. Human brain and lung mast cells were successfully extracted from immune-humanized mice and cultured for analysis. Gene expression analysis confirmed that human mast cell-specific genes were expressed. Furthermore, human histamine release by IgE stimulation and human IL-13 production by human IL-33 stimulation were confirmed. Based on the results of this study, we plan to further investigate the molecular pathogenesis of stress-related asthma using immune-humanized mice.
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| Free Research Field |
呼吸器内科学 心身医学
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| Academic Significance and Societal Importance of the Research Achievements |
野生型マウスにおいてストレス関連喘息の病態に関与する遺伝子群を同定することができたこと、さらに免疫ヒト化マウス由来ヒトマスト細胞は、肺および脳ともにヒトマスト細胞の表現型を有していることなどを見出すことができたことは、ヒトストレス関連喘息の分子病態を解明するために有用であり、学術的意義があると考える。これらは、ストレス関連喘息の新たな診断バイオマーカー、治療標的を見出すことができる基盤となる成果であり、社会的意義もあると考える。
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