2023 Fiscal Year Final Research Report
Establishment of a Preservation Method for Mesenchymal Stem Cell-Derived Extracellular Vesicles and Development of a Novel Treatment for Acute Liver Failure
Project/Area Number |
21K07998
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 53010:Gastroenterology-related
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Research Institution | Yamagata University |
Principal Investigator |
Haga Hiroaki 山形大学, 医学部, 准教授 (70466613)
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Co-Investigator(Kenkyū-buntansha) |
星川 恭子 山形大学, 医学部, 助教 (20613053)
勝見 智大 山形大学, 医学部, 助教 (70637355)
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Project Period (FY) |
2021-04-01 – 2024-03-31
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Keywords | 細胞外小胞 / 間葉系幹細胞 / 急性肝不全 / Extracellular Vesicles |
Outline of Final Research Achievements |
In mice with acute liver failure, mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have been shown to improve liver function and liver tissue. However, in urgent clinical settings, it is necessary to establish an effective preservation method for MSC-EVs. We investigated the lyophilization method for preserving MSC-EVs and studied the effects of lyophilized MSC-EVs on mice with acute liver failure. By comparing lyophilization methods using PBS, 1% sucrose solution, and 5% sucrose solution, it was demonstrated that the number, RNA, and morphology of MSC-EVs were best maintained with the 5% sucrose solution. Furthermore, lyophilized MSC-EVs in 5% sucrose solution were confirmed to improve liver function and liver tissue in mice with acute liver failure, similarly to non-lyophilized MSC-EVs.
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Free Research Field |
肝臓病学
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Academic Significance and Societal Importance of the Research Achievements |
昏睡型急性肝不全は、死亡率が極めて高い疾患であり、肝臓移植を要する患者も存在する。しかし、深刻なドナー不足のため、新しい肝再生医療の開発が必要とされる。急性肝不全マウスにおいて、MSC-EVは肝機能改善に寄与するが、臨床応用を考えた際に問題となるのはMSC-EVの保存方法である。間葉系幹細胞の培養液からMSC-EVを抽出するには時間がかかるため、緊急性を有する臨床においてはMSC-EVの有効な保存方法の確立が必要である。今回、我々はMSC-EVの凍結乾燥法の確立と、その凍結乾燥MSC-EVが急性肝不全マウスの改善に寄与することを示した。これにより、EVの臨床応用に一歩近づくものと考えられる。
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