2023 Fiscal Year Final Research Report
Analysis of IL-36 cascade on pustular diseases
| Project/Area Number |
21K08298
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 53050:Dermatology-related
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
高橋 聡文 滋賀医科大学, 医学部, 講師 (70630862)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | IL-36 / IL-36RN / HaCaT / IL36Ra |
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| Outline of Final Research Achievements |
We looked for various conditions in which qPCR expression of IL36RN mRNA in HaCaT increased, but significant increase was not observed by the stimulation such as TNF-a+IL-17A, IL-1β, and LPS. We generated HaCaT cell line in which IL36RN gene was knocked out using lentiCRISPR v2. We stimulated the knocked out HaCaT cell with IL-1β, Dermcidin-1L, Poly(I:C), and LPS, and compared the expression of TNF-α, IL-6, and IL-8 with normal HaCaT cell, but significant difference was not observed.
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| Free Research Field |
膿疱症
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| Academic Significance and Societal Importance of the Research Achievements |
培養表皮角化細胞であるHaCaT細胞におけるIL-36カスケードに注目した限りにおいては、IL-36レセプターアンタゴニストであるIL36Raが TNF-α、IL-6、IL-8の産生に果たす役割は大きいとは言えない結果であった。
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